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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,275)
Mouse Secondary Antibodies (14,507)
Rabbit Secondary Antibodies (6,327)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,240)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (55)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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1,426 – 1,440 of 72,126 results
1,426

Donkey anti-Sheep IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate Janelia Fluor 525
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry (Paraffin)
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Sheep IgG (H+L) Secondary Antibody was developed against sheep IgG-heavy and light chain.
Primary or Secondary Secondary
1,427

Donkey anti-Rabbit IgG Fc Secondary Antibody, DyLight 350, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 350
Applications ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Antigen IgG Fc
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50 mM Sodium Borate
Classification Polyclonal
Immunogen Rabbit IgG F(c) fragment
Primary or Secondary Secondary
1,428

Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Goat
Host Species Donkey
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Goat IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
1,429

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Simple Western,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200 - 1:5000, ELISA 1:200 - 1:5000, Immunohistochemistry 1:500 - 1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was developed against purified rabbit IgG (H+L).
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep.
1,430
Promotions Available

Goat IgG VisUCyte HRP Polymer, R&D Systems™
SDP

Donkey Polyclonal Antibody has been used in 2 publications

Content And Storage 6 months from date of receipt, 2 to 8 degreesC as supplied.
Host Species Donkey
Conjugate HRP Polymer
Applications Immunohistochemistry
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Immunohistochemistry
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied as a solution in PBS with preservative.
Immunogen Goat IgG
Classification Polyclonal
Primary or Secondary Secondary
Test Specificity Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
1,432

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 594
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunocytochemistry/Immunofluorescence, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins
1,433

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 350, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Donkey
Conjugate DyLight 350
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Primary or Secondary Secondary
1,434

Donkey anti-Goat IgG (H+L) Secondary Antibody, PE (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 3 publications

Content And Storage Store at 4C in the dark.
Target Species Goat
Host Species Donkey
Conjugate PE
Applications Dot Blot,Flow Cytometry,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Concentration 0.5 mg/mL
Antigen IgG (H+L)
Gene Symbols IGHG1
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot, Flow Cytometry 1:100 - 1:250, Immunocytochemistry/Immunofluorescence 1:100 - 1:250, Dot Blot
Classification Polyclonal
Immunogen Goat IgG whole molecule
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
1,435

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 594
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG-heavy and light chain.
Primary or Secondary Secondary
1,436

Donkey anti-Rat IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C in the dark.
Target Species Rat
Host Species Donkey
Conjugate Fluorescein
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Rat IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rat IgG and Rat Serum.
1,437

Invitrogen™ Donkey anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 594

Donkey Polyclonal Secondary Antibody

Content And Storage 4°C
Target Species Human
Host Species Donkey
Conjugate DyLight 594
Applications Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 0.5 mg/mL
Antigen Human IgG (H+L) Cross-Adsorbed
Regulatory Status RUO
Purification Method Antigen affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 0.2% BSA and 0.09% sodium azide; pH 6.8 to 7.4
Immunogen Human IgG-heavy and light chain.
Classification Polyclonal
Primary or Secondary Secondary
1,438

Donkey anti-Human IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Human IgG (H+L) Secondary Antibody was developed against human IgG-heavy and light chain.
Primary or Secondary Secondary
1,439

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 488, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 488
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG-heavy and light chain.
Primary or Secondary Secondary
1,440

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Sheep
Host Species Donkey
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:2000 - 1:20000, ELISA 1:2000 - 1:20000, Immunohistochemistry 1:500 - 1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Sheep IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins. This antibody has been pre-adsorbed against human and rabbit IgG.